Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
* Modification: Add 0.3g/L nitrate to the basic medium, and increase the amount of sulfide used. Grow the strain on agar (e.g., agar slants) overlayed with a small amount of sterile tap water.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaNO3* | 0.30 | g/l | - | - | |
NH4Cl | 0.20 | g | 0.2 | 3.739 | |
K2HPO4 | 0.01 | g | 0.01 | 0.057 | |
MgSO4 x 7 H2O | 0.01 | g | 0.01 | 0.041 | |
CaSO4
(saturated solution) |
20.00 | ml | - | - | |
Trace element solution | 5.00 | ml | - | - | |
Na-acetate | 0.10 | g | 0.1 | 1.219 | |
Agar , if required | 12.00 | g | 12 | 35.679 | |
Distilled water | 1000.00 | ml | - | - | |
1 Adjust pH to 7.5 before autoclaving. Sterilize separately a 10% (w/v) solution of Na2S x 9 H2O; add the following amount of this solution to the medium after autoclaving (= shortly before using the medium). | |||||
neutralized Na2S x 9 H2O solution, 10% (w/v)* | 7.50 | ml | - | - | |
2 Note: For small volumes, such as 5 mL agar slants, you can use the respective volume of a sterile neutralized 3% Na2S x 9 H2O solution (e.g., 50µL/5 mL = 0.3g/L) |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
EDTA | 0.20 | g | 0.2 | 0.684 |
FeSO4 x 7 H2O | 0.70 | g | 0.7 | 2.518 |
ZnSO4 x 7 H2O | 0.01 | g | 0.01 | 0.035 |
MnSO4 x 4 H2O | 2.00 | mg | 0.002 | 0.009 |
CuSO4 x 5 H2O | 5.00 | µg | 5.0e-6 | 2.0e-5 |
H3BO3 | 10.00 | mg | 0.01 | 0.162 |
Co(NO3)2 | 1.00 | mg | 1.0e-3 | 0.005 |
Na2MoO4 x 2 H2O | 1.00 | mg | 1.0e-3 | 0.004 |
Distilled water | 1000.00 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Na2S x 9 H2O | 3 | %(w/v) | 30 | 124.905 | |
Distilled water | 1000 | ml | - | - | |
1 Adjust pH to 7.0. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Na2S x 9 H2O | 10 | g | - | - | |
Distilled water | 100 | ml | - | - | |
1 Adjust pH to 7.0. |
Last modified: | 22.02.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Defined medium |
Final pH: | 7.5 |
Edit in Medium builder |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Agar | 12 | 35.679 |
Na2S x 9 H2O | 0.3 | 1.249 |
NH4Cl | 0.2 | 3.739 |
Na-acetate | 0.1 | 1.219 |
K2HPO4 | 0.01 | 0.057 |
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